Fluorescence Spectrophotometric Determination of 6- Thioguanine in Biological Sample
نویسندگان
چکیده
Present work describes the fluorescence spectroscopy determination of (6-TG) using silver nanoparticles (AgNPs). The acridine could be electrostatically adsorbed to the surface of the citrate-stabilized silver nanoparticles. Upon addition of 6-TG, the acridine is replaced from the surface of AgNPs which induce the aggregation of AgNPs. Under optimum condition, the fluorescence intensity enhance showed the linear relationships with 6-TG concentration range from 0.05 × 10-8 M to 4.0 × 10-7 M. The detection limits (3s) and % RSD for 6-TG was occurred to be 9.6 nM, ± 2 % respectively. Proposed work has been successfully applied for the determination of 6-TG in biological sample such as urine sample. KeywordsFluorescence spectroscopy, silver nanoparticles, acridine, 6-Thioguanine.
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